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BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3)

Cat no: B2199-97A

BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3)

BNIP3 (Bcl-2/E1B-19kD interacting prote in 3) is a pro-apoptotic mitochondrial protein and Bcl-2 family member that contains a Bcl-2 homology 3 (BH3) domain a nd a carboxyl terminal transmembrane (TM) domain (1-3). While BNIP3 has a predicted molecular weight of about 22 kD , it runs anomalously on SDS-PAGE and includes a band of around 60 kD that may be a dimeric form that is not re- duced (2). BNIP3 associates with anti-apoptotic family members Bcl-2, Bcl-xL, and the adenovirus homologue E1B-1 9kD. BNIP3 is distinct from other Bcl-2 family members t hat contain only the BH3 domain in that the TM domain, and- not the BH3 domain, is required for mitochondrial targetin- g and pro-apoptotic activity (4). In addition to apoptosis, BNIP3 has been implicated in necrosis (5) and autophagy (6,7), although the relationship between BNIP3 and these processes is not fully understood. Increased expression of BNIP3 under hypoxic conditions is mainly regulated by the transcription factor HIF-1a (8-10). Silencing of the BNIP3 promoter by methylation has been observed in several types of cancer cells and may play an important role in their survival (11-14). \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2199-97A

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Affinity chromatography.

Isotype

IgG

References

(1) Boyd, J.M. et al. (1994) Cell 79, 341

Additional Info

Detects endogenous levels of total BNIP3 protein from mouse and rat.

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