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Caspase 2, Apoptosis Detection, Fluorometric BioAssay(TM) Kit (ICH1, Nedd2)

Cat no: C2086-75

Caspase 2, Apoptosis Detection, Fluorometric BioAssay(TM) Kit (ICH1, Nedd2)

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2/ICH-1 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by ICH-1 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity. \n\nComponents: 25 assays 100 assays\nCell Lysis Buffer 25ml 100ml\n2X Reaction Buffer 2ml 4 x 2ml\nVDVAD-AFC Substrate (1mM) 125ul 500ul\nDTT (1M) 100ul 400ul\n\nStability:\nStore kit at -20 degrees C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4 degrees C after opening). All reagents are stable for 1 year under proper storage conditions.

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SPECIFICATIONS

Catalog Number

C2086-75

Size

25Tests

References

Product Specific:\nLi J., et al. (2002) J. Biol. Chem. 277:49504-49510.\n\nGeneral:\n1. Talanian, R.V., et al., J. Biol.Chem. 272:9677-9682 (1997)\n2. Wang, L., et al., Cell 78:739-750 (1994)

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