Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase 9 Apoptosis Detection, Colorimetric BioAssay(TM) Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity.
Kit Components: 25 assays 100 assays
C2088-10A: Cell Lysis Buffer 1 x 25ml 1 x 100ml
C2088-10B: 2X Reaction Buffer 1 x 2ml 4 x 2ml
C2088-10C: LEHD-pNA Substrate (4mM) 1 x 125ul 1 x 500ul
C2088-10D: DTT (1M) 1 x 100ul 1 x 400ul
C2088-10E: Dilution Buffer 1 x 25ml 1 x 100ml
Storage and Stability:
Store kit at -20 degrees C. Store Cell Lysis Buffer (C2088-10A), 2X Reaction Buffer (C2088-10B) and Dilution Buffer (C2088-10E) at 4 degrees C after opening. Note: Protect LEHD-pNA (C2088-10C) from light. All reagents are stable for 6 months.
Quick Search
Products 
