Caspase Inhibitor I (Z-VAD (OMe)-FMK)

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.\n\nSequence:\nZ-Val-Ala-Asp(OMe)-CH2F\n\nSolubility:\nDMSO (50mM)\n\nMolecular Weight:\n~467.5\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

029435

Size

1mg

Form

Supplied as a white solid.

Purity

Single spot by TLC

References

1. Heng, B.C., et al. 2007. Biosci. Rep. 27, 257. 2. Martin, U., et al. 2007. Apoptosis 12, 525. 3. Misaghi, S., et al. 2004. Chem. Biol. 11, 1677. 4. Joo, C., et al. 1999. Ophthalmic Res. 31, 287. 5. McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229. 6. Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312. 7. Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735. 8. An, S. and Know, K.A. 1996. FEBS Lett. 386, 115. 9. Cain, K., et al. 1996. Biochem J. 314, 27. 10. Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266. 11. Pronk, G.J., et al. 1996. Science 271, 808. 12. Slee, E.A., et al. 1996. Biochem. J. 315, 21. 13. Chow, S.C., et al. 1995. FEBS Lett. 364, 134. 14. Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283. 15. Zhu, H., et al. 1995. FEBS Lett. 374, 303. 15. Heng, B.C., et al. 2007. Biosci. Rep. 27, 257. 16. Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691. 17. Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.