CD16 (Fc gamma RIII alpha, NK cells)

Supplier:

United States Biological

Cat no: C2267-56

CD16 (Fc gamma RIII alpha, NK cells)

CD16 (also known as low affinity Fc gamma receptor III for IgG (FcgRIII) or Leu11) exists as a polypeptide-anchored form (FcgRIIIA or CD16A) on human natural killer (NK) cells and monocytes/macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcgRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2. CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions: \nImmunohistochemistry (Formalin/paraffin): 1:10-1:20; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2267-56

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1. Knapp, W., et al., (eds) Leukocyte Typing IV, Oxford University Press, Oxford (1989). 2. Lanier, L.L., et al., J. Exp. Med. 162(6): 2089-2106 (1985). 3. Nagarajan, S., et al., J. Biol. Chem. 270(43): 25,762-25,770 (1995). 4. McKenzie, S.E. & Schreiber, A.D., Curr. Opin. Hematol. 1(1): 45-52 (1994). 5. Cerboni, C., et al., Eur. J. Immunol. 28(3): 1005-1015 (1998). 6. Braakman, E., et al., Cell Immunol. 143: 97-107 (1992).

Additional Info

Recognizes human CD16 at 50-65kD.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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