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CD274 (CD274 Molecule, B7 Homolog 1, B7H1, B7-H1, B7-H, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1)

Cat no: C2549-22P1

CD274 (CD274 Molecule, B7 Homolog 1, B7H1, B7-H1, B7-H, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1)

CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection. CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma is associated with poor prognosis. Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival. Gamma Interferon and PHA can up regulate CD274 expression on T cells. \n\nApplications:\nSuitable for use in ELISA and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 5ug/ml (80ul)\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol:\n1. Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5ug/ml PHA.\n2. Five x10e5 cells/tube were incubated 45 minutes on ice with 80ul of C2549-22P1 at 5ug/ml. \n3. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed twice, fixed and analyzed by FACS using a lymphoid gate. \n4. Cells stained positive with a mean shift of 0.33 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.\n5. Binding was blocked when reagent was pre incubated with a 10-fold excess of C2549-22R.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2549-22P1

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS. Preservative free.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Cancer Research (April 2006) 66:3381, 2. J Molecular Medicine (Feb 2004) 81(5):281, 3. Int J Hematol (Nov 2003) 78(4):321.

Additional Info

Recognizes human CD274 (B7-H1). Binds to recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.\n

Alternative Names

MGC142294, MGC142296

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