CNG1, Rat (Cyclic Nucleotide-Gated Channels 1) Control Peptide

Control Peptide for C5999 (antiserum) and C5999-05 (affinity purified antibody).\n\nThe cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated alpha and beta subunits. Several CNG a-subunits (CNG alpha 1-3) and b-subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves. b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a and b subunits.\n\nBovine CNG1 was initially cloned and characterized from rod cells. CNG1 (rat 683aa, mouse 684aa, human 686aa; also describes as OCNC1) is primarily expressed in outer segment of photoreceptor rod cells, and in a variety of tissues (kidney, eye, pineal gland, pituitary, adrenal, spleen, brain, heart, skeletal muscle and testis). Human/mouse CNG1 exhibit ~88% sequence homology.\n \nApplications: \nSuitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml\nAntibody Blocking: 5-10ug per 1ul C5999 (antiserum) or 1ug C5999-05 (affinity purified antibody).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5999-10

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide

Purity

Highly purified

References

1. Ding C et al (1997) Am. J. Physiol. 272, C1335. 2. Pittler SJ et al (1992) J Biol. Chem. 267, 6257. 3. Karlson KH et al (1995) BBA 1236, 197. 4. Dhallan RS et al (1992) J Neurosci. 12, 3248. 5. Distler M et al (1994) Neuropharmacol. 33, 1275. 6. Bonigk et al (1993) Neuron 10, 865.

Additional Info

Synthetic peptide corresponding to 16aa sequence near N-terminus of rat CNG1. No significant sequence homology is detected with other CNGs. Species Sequence Homology: mouse: 93%; human, canine, bovine, porcine: 87%; chicken: 56%.