COX 1, Murine cDNA Probe (Cyclooxygenase-1, PGHS-1, Prostaglandin Endoperoxide Synthase-1, PHS 1, Ovarian Cancer Marker), BioGenomics(TM)

The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The biosynthesis of PGs and some other prostanoids, is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachiodonic acid to prostaglandin/prostanoid precursor PGH2. Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified. COX1, a constitutively expressed isoform, produces physiologically relevant prostanoids such as those in stomach and platelets. COX2 isoform is inducible and rapidly upregulated at inflamation sites and forms proinflamatory prostanoids. The overexpression of COX-2 also leads to tumerogenesis. Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported. Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kD in human aorta) and PCOX1b have also been characterized. The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity. The nonsteroidal antiinflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3), This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2.\n\nSize of the cDNA:\n1.7Kb.\n\nApplications:\nSuitable for use for the synthesis, by standard random priming or nick translation techniques, of radiolabeled probes for investigations of prostaglandin H synthase gene expression. Hybridizes to mRNA on Northern blot 2.7-3.0Kb in length. Other applications not tested.\n\nOptimum dilutions to be determined by researcher.\n\nStorage:\nCan be stored for at least one year at -70 degrees C. Avoid repeated freeze/thaw cycles.

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SPECIFICATIONS

Catalog Number

C7904-78A

Size

1ug

Applications

WB

Form

Supplied in TE buffer.

References

1. Casey, M.L., et al., J. Biol. Chem., 263 : 7846 (1988)2. Wu, K.W., et al., J. Biol. Chem., 263 : 19043 (1988)3. Bailey, J.M., et al., Biochem. Biophys. Res. Comm., 157: 1159(1988)4. Masferrer, J.L., et al., J. Clin. Invest., 86 : 1375 (1990)5. DeWitt, D.L., Biochem. Biophys. Acta., 1083 : 121 (1991)