CTNNB, Recombinant, Human, 6-His-tag (Catenin beta-1, Beta-catenin, CTNNB1, OK/SW-cl.35, PRO2286)

B-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of b-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of b-catenin have been implicated in a number of human cancers.\n\nSource:\nRecombinant corresponding to full length human b-catenin, fused to 6His-tag at N-terminal c-Myc, expressed by baculovirus in Sf21 insect cells.\n\nMolecular Weight: \n~91kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029448

Size

10ug

Form

Supplied as a liquid in 25mM MOPS pH7.5, 5mM MgCl2, 0.01% Tween 20, 1mM ATP.

Purity

~86% (SDS-PAGE, Coomassie blue staining)

References

1. Ying Y. and Tao Q. Epigenetic Disruption of the WNT/b-catenin Signaling Pathway in Human Cancers. Epigenetics, 4: 307-312, 2009. 2. MacDonald B. T. et al., WNT/b-catenin Signaling: Components, Mechanisms, and Diseases. Dev Cell, 17:9-26, 2009. 3. Bullions L.C. and Levine A.J. The Role of b-catenin in Cell Adhesion, Signal Transduction, and Cancer. Curr Opin Oncol. 10: 81-87, 1998. 4. Latres E. et al., The Human F box protein b-TrCP Associates with the Cul1/Skp1 Complex and Regulates the Stability of b-catenin. Oncogene, 18: 849-854, 1999. 5. Orford K. et al., Serine Phosphorylation-Regulated Ubiquitination and Degradation of b-catenin. J Biol Chem, 272: 24735-24738, 1997. 6. Kitagawa M. et al., An F-box Protein, FWD1, Mediates Ubiquitin Dependent Proteolysis of b-catenin. The EMBO Journal, 18: 2401-2410, 1999.