CYTIP (PSCDBP, Cytohesin-interacting Protein, Cytohesin Binder and Regulator, CYBR, Cytohesin-associated Scaffolding Protein, CASP, Cytohesin-binding Protein HE, Cbp HE, Pleckstrin Homology Sec7 and Coiled-coil Domains-binding Protein)

Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK \nMacrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. \n \nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000 \nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9096-06A

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Coppola,V., et al., (2006) Mol. Cell. Biol. 26 (14), 5249-5258. 2. Watford,W.T., Li,D., Agnello,D., Durant,L., Yamaoka,K., Yao,Z.J., Ahn,H.J., Cheng,T.P., Hofmann,S.R., Cogliati,T., Chen,A., Hissong,B.D., Husa,M.R., Schwartzberg,P., O'Shea,J.J. and Gadina,M. (2006) Cytohesin binder and regulator (cybr) is not essential for T- and dendritic-cell activation and differentiation. Mol. Cell. Biol. 26 (17), 6623-6632. 3. Boehm,T., Hofer,S., Winklehner,P., Kellersch,B., Geiger,C., Trockenbacher,A., Neyer,S., Fiegl,H., Ebner,S., Ivarsson,L., Schneider,R., Kremmer,E., Heufler,C. and Kolanus,W. (2003) Attenuation of cell adhesion in lymphocytes is regulated by CYTIP, a protein which mediates signal complex sequestration. EMBO J. 22 (5), 1014-1024. 4. Kitano,J., Kimura,K., Yamazaki,Y., Soda,T., Shigemoto,R., Nakajima,Y. and Nakanishi,S. (2002) Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins. J. Neurosci. 22 (4), 1280-1289.

Additional Info

Recognizes endogenous and over-expressed Cybr at ~40kD. Species sequence homology: rat