Keratin is a family of about 20 proteins that form intermediate filament in epithelial cells. Obligate heteropolymers of a type I and a type II subunit, they contribute to the structure and strength of the cytoskeleton. Mutations in keratin genes cause keratinopathies, inherited diseases of the skin and its appendages. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant intermediate filament protein complement in an epithelial cell-specific manner. Cytokeratin 14 belongs to the acidic or type I keratin family. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in this gene results in epidermolysis bullosa simplex, epidermolysis bullosa simplex Dowling-Meara type, epidermolysis bullosa simplex Weber-Cockayne type, and Naegeli-Franceschetti-Jadassohn syndrome. It is strongly expressed in the outer root sheath of anagen follicles and found in keratinocytes surrounding the club hair during telogen. It is a prototypic marker of dividing basal keratinocytes where its gene is transcribed at high levels. It is encoded on chromosome 17q12-q21.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:50-1:100
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:75
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Positive Control:
Skin
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
