Cytokeratin 20, Recombinant, Human

Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. The recently identified cytokeratin-20 (CK-20) was found with immunohistochemical methods to be expressed in gastrointestinal epithelium, uroepithelial cells, and Merkel cells. Clues were also found for low expression of CK-20 in endometrial carcinoma cells\n\nRecombinant Human Cytokeratin 20 is a single, non-glycosylated polypeptide chain having a molecular mass of 48,553 Daltons.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a calibrated solution of cytokeratin 20 as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized CK-20 in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C9097-29R

Size

5ug

Form

Supplied as a lyophilized powder in 30mM Tris-HCl, pH 8.0, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Chen, Z.M. & Wang, H.L., Am. J. Surg. Pathol. 28(10): 1352-1359 (2004). 2. Franchi, A., et al., Virchows Arch. 445(1): 63-67 (2004). 3. McGregor, D.K., et al., Am. J. Surg. Pathol. 28(6): 712-718 (2004). 4. Tot, T., Med. Sci. Monit. 10(5): BR139-BR143 (2004). 5. Zou, S.M., et al., Zhonghua Yi Xue Za Zhi 83(15):1350-1352 (2003). 6. Ramos-Vara, J.A., et al., Vet. Pathol. 40(1): 55-62 (2003).