Cytokeratin 2e

This clone represents an excellent marker to study terminal epidermal differentiation. \n\nApplications: \nSuitable for use in Immunoblot (Western) and Immunohistochemistry.\n\nRecommended Dilution:\nImmunohistochemistry: 1:150\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-33H

Size

1ml

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Xen/Amph

Form

Supplied as a liquid.

P Type

Mab

Purity

Hybridoma culture supernatant

Isotype

IgG1

References

1. Collin C., Moll R., Kubicka S., Ouhayoun J.-P. and Franke W.W. (1992). Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthsized late during differentiation, Exp. Cell Res. 202: 132-141.\n2. Swensson O, Langbein L, McMillan JR, Churchill LJ, Leigh IM, McLean WHI, Lane EB, Eady RAJ. Specialized keratin expression pattern in human ridged skin as an adaption to high physical stress. Br.J.Dermatol. 139, 767-775 (1998)

Additional Info

Antigen recognized in species (tested so far) Only reactive on human tissue; bovine, mouse, rat, Xenopus laevis are negative. Reactive with epidermal cells in uppermost supra-basal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). It is also reactive on hyperkeratosis of diverse viral and genetic origin. The Mab does not react with palate cytokeratin 2p.