Cytokeratin (FITC)

Used for the localization of cytokeratins using various immunochemical assays including direct immunofluorescence using frozen or fixed tissue sections or cultured cells. It may also be used in flow cytometry of normal and neoplastic epithelial cells. Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells.\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:25.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C9097-01U

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Fel, Goat, GP, Prc, Rabbit, Sheep

Form

1% BSA, 0.01M PBS. Label with FITC.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Lane EB et al., (1990)Semin Cancer Biol 1:165-79. 2. Moll R et al. (1982) Cell 31:11-24.

Additional Info

Recognizes the 58kD (cytokeratin 5), the 56kD (cytokeratin 6) and the 52kD (cytokeratin 8) bands in immunoblotting. Cytokeratin is a broad spectrum antibody which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. Species Crossreactivity: cytokeratins from many species (e.g., rabbit, guinea pig, goat, bovine, sheep, rat, mouse, hamster, canine, cat, chicken, viper, lizard and carp).