Cytokeratin, HMW (High Molecular Weight Keratin)

Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells in a cell-specific manner. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant IF protein complement in an epithelial cell-specific manner. Most of the type I cytokeratin consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. and the type II cytokeratin are clustered in a region of chromosome 12q12-q13.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry(Paraffin): 1:200-1:400. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138537

Size

20ug

Applications

ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Woodcock et al. J Cell.Biol 95: 580, 1982. 2. Tseng et al. Cell 30: 361, 1982. 3. Sun et al. J Invest Dermatol 86: 249, 1986. 4. Klein-Szanto et al. Arch Pathol Lab Med 111; 1057, 1987.

Additional Info

Recognizes human cytokeratin. Recognizes most of basic (type II) keratins. Recognizes keratin polypeptides of 58, 65-67kD. In keratinized epidermis, 58kD keratin is present in the basal and suprabasal layers, 65-67kD keratin is present in cells above the basal layers. Can be used to stain different layers in an epithelial tissue and various neoplasms of epithelial origin.