Cytokeratins belong to the intermediate filament family of proteins and are classified according to their type. Type I Cytokeratins are low molecular weight and acidic whereas Type II Cytokeratins are higher weight and basic to neutral. This antibody broadly detects Cytokeratins 1, 4, 5, 6, 8, 10, 13, 18 and 19. Expression of these Cytokeratins is frequently tissue specific.
Applications:
Suitable for use in Western Blot and Immunohisochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry (Paraffin and Formalin): 2.0-5.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.