DC-SIGN (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1, Dendritic Cell-specific ICAM-3-grabbing Non-integrin)

Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Adhesion Blockade and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry (Immunophenotyping): 10ul of 25-50ug/ml labels up to 10e6 cells Adhesion Blockade: 5ug/ml (5x10e6 cells/well). Immunohistochemistry: 25ug/ml detects human DC-SIGN in fixed cells and tissue sections. Western Blot: 1-2ug/ml The detection limit is a~5ng/well under non-reducing and reducing conditions using a colorometric detection system,. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Optimal dilutions to be determined by the researcher. Positive Control: Transfected NIH/3T3 cells and monocyte derived dendritic cells. Storage and Stability: Lyophilized powder may be stored at -20 degrees C for short-term only. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.