DC-SIGN (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1, Dendritic Cell-specific ICAM-3-grabbing Non-integrin)

Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry, Adhesion Blockade and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry (Immunophenotyping): 10ul of 25-50ug/ml labels up to 10e6 cells \nAdhesion Blockade: 5ug/ml (5x10e6 cells/well).\nImmunohistochemistry: 25ug/ml detects human DC-SIGN in fixed cells and tissue sections.\nWestern Blot: 1-2ug/ml The detection limit is a~5ng/well under non-reducing and reducing conditions using a colorometric detection system,. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTransfected NIH/3T3 cells and monocyte derived dendritic cells.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C for short-term only. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D1550-03G

Size

500ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2, 5% trehalose. Reconstitute in 1ml sterile 40-50% glyercol, PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography. Endotoxin: (same/less than)0.1 EU/ug (LAL).

Isotype

IgG2b

References

1. Geijtenbeek, T.B.H. et al., 2000, Cell 100:575. 2. Geijtenbeek, T.B.H. et al., 2000, Nature 1:353. 3. Geijtenbeek, T.B.H. et al., 2000, Cell 100:587. 4. Curtis, B.M. et al., 1992, Proc. Natl. Acad. Sci. USA 89:8356.5. Pohlman, S. et al., 2001, J. Virol. 75:4664. 6. Mummidi, S. et al., 2001, J. Biol. Chem., 2001, May 3 [epub ahead of print].

Additional Info

Recognizes human DC-SIGN on transfected NIH/3T3 cells and on monocyte derived dendritic cells at 44kD. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

Alternative Names

Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1