Home  >  Products  >  DDB2 (Damage-specific DNA Binding Protein 2, DNA Damage-binding Protein 2, DDB p48 Subunit, DDBb, FLJ34321, UV-damaged DNA-binding Protein 2, UV-DDB 2, UV-DDB2)
DDB2 (Damage-specific DNA Binding Protein 2, DNA Damage-binding Protein 2, DDB p48 Subunit, DDBb, FLJ34321, UV-damaged DNA-binding Protein 2, UV-DDB 2, UV-DDB2)

DDB2 (Damage-specific DNA Binding Protein 2, DNA Damage-binding Protein 2, DDB p48 Subunit, DDBb, FLJ34321, UV-damaged DNA-binding Protein 2, UV-DDB 2, UV-DDB2)

Cat no: D1584-80B

Supplier: United States Biological
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Damaged DNA-Binding Protein (DDB) consists of a 127kD subunit (DDB1) and a 48kD subunit (DDB2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB) (1-3). In conjuction with CUL4A and ROC-1, the UV-DDB complex forms an E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway (4-6). Loss of DDB activity has been identified in a subset of xeroderma pigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients (7-10). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D1584-80B
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100 microg/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
Alternative names: Damage-specific DNA Binding Protein 2, 48kDa
References: (1) Reardon, J.T. et al. (1993) J Biol Chem 268, 21301-8. (2) Keeney, S. et al. (1993) J Biol Chem 268, 21293-300. (3) Hwang, B.J. and Chu, G. (1993) Biochemistry 32,1657-66. (4) Chu, G. and Chang, E. (1990) Proc Natl Acad Sci USA 87, 3324-7. (5) Hirschfeld, S. et al. (1990) Mol Cell Biol 10, 2041-8. (6) Payne, A. and Chu, G. (1994) Mutat Res 310, 89-102. (7) Chu, G. and Chang, E. (1988) Science 242, 564-7. (8) Nichols, A.F. et al. (1996) J Biol Chem 271, 24317-20. (9) Kataoka, H. and Fujiwara, Y. (1991) Biochem Biophys Res Commun 175, 1139-43. (10) Keeney, S. et al. (1992) Mutat Res 273, 49-56.
Additional info: Recognizes endogenous levels of total DDB2. Species Crossreactivity: monkey, mouse, rat.

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