DDB2 (Damage-specific DNA Binding Protein 2, DNA Damage-binding Protein 2, DDB p48 Subunit, DDBb, FLJ34321, UV-damaged DNA-binding Protein 2, UV-DDB 2, UV-DDB2)

Damaged DNA-Binding Protein (DDB) consists of a 127kD subunit (DDB1) and a 48kD subunit (DDB2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB) (1-3). In conjuction with CUL4A and ROC-1, the UV-DDB complex forms\nan E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway (4-6). Loss of DDB activity has been identified in a subset of xeroderma pigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients (7-10).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

D1584-80B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100 microg/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Reardon, J.T. et al. (1993) J Biol Chem 268, 21301-8. (2) Keeney, S. et al. (1993) J Biol Chem 268, 21293-300. (3) Hwang, B.J. and Chu, G. (1993) Biochemistry 32,1657-66. (4) Chu, G. and Chang, E. (1990) Proc Natl Acad Sci USA 87, 3324-7. (5) Hirschfeld, S. et al. (1990) Mol Cell Biol 10, 2041-8.\n(6) Payne, A. and Chu, G. (1994) Mutat Res 310, 89-102. (7) Chu, G. and Chang, E. (1988) Science 242, 564-7. (8) Nichols, A.F. et al. (1996) J Biol Chem 271, 24317-20. (9) Kataoka, H. and Fujiwara, Y. (1991) Biochem Biophys Res Commun 175, 1139-43. (10) Keeney, S. et al. (1992) Mutat Res 273, 49-56.

Additional Info

Recognizes endogenous levels of total DDB2. Species Crossreactivity: monkey, mouse, rat.

Alternative Names

Damage-specific DNA Binding Protein 2, 48kDa