DDX4, NT (DDX4, VASA, Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog) (Biotin)

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

034555-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Tilgner, K., et al. Stem Cells 28(1):84-92(2010)\nSugimoto, K., et al. J. Hum. Genet. 54(8):450-456(2009)\nHashimoto, H., et al. Gynecol. Oncol. 111(2):312-319(2008)\nAlbamonte, M.S., et al. Hum. Reprod. 23(8):1895-1901(2008)\nGuo, X., et al. Asian J. Androl. 9(3):339-344(2007)

Additional Info

Human, mouse

Alternative Names

DDX4, VASA, Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog