Dectin 2, Recombinant, Human (CLEC6A, CLECSF10, NKCL)

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% aa sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced b isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kD molecule that is expressed on monocytes, tissue macrophages, and activated CD4+ T cells (4-6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor g subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the g subunit, activation of NFkB, and enhanced release of TNF-a and IL-1ra (8). Macrophage Dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4+ CD25+ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-b (10).\n\nSource: \nHuman CD33 Signal Peptide (Met 1-Ala 16), HHHHHH, Human Dectin-2 (Thr 46-Leu 209). A DNA sequence encoding the signal peptide from human CD33 was fused to the N-terminal 6X histidine-tagged extracellular domain of human Dectin-2 (Thr 46-Leu 209) (Accession # NP_001007034; Q6EIG7). The chimeric protein was expressed in a mouse myeloma cell line, NS0.\n\nMolecular Mass: \nBased on N-terminal amino acid sequencing, the recombinant human Dectin-2 begins with the histidine tag. It has a predicted molecular mass of approximately 19.7 kD. As a result of glycosylation, the recombinant human Dectin-2 migrates as an approximately 23 kD protein in SDS-PAGE under reducing conditions.\n\nEndotoxin Level: \n< 1.0 EU per 1 microg of the protein as determined by the LAL method.\n\nActivity: \nMeasured by its ability to bind biotinylated a-D-Mannose-PAA in a functional ELISA.\n\nReconstitution: \nIt is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.\n\nStorage and Stability: \nLyophilized samples are stable for up to twelve months at -20 degrees C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degrees C for one month or at -20 degrees C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

D1876-50

Size

50ug

Form

Supplied as a lyophilized powder in PBS.

Purity

(same/more than) 90%, as determined by SDS-PAGE and visualized by silver stain.

References

1. Kanazawa, N., 2007, J. Dermatol. Sci. 45:77. \n2. Kanazawa, N. et al., 2004, Immunobiology 209:179. \n3. Flornes, L.M. et al., 2004, Immunogenetics 56:506. \n4. Kanazawa, N. et al., 2004, J. Invest. Dermatol. 122:1522. \n5. Gavino, A.C. et al., 2005, Exp. Dermatol. 14:281. \n6. Taylor, P.R. et al., 2005, Eur. J. Immunol. 35:2163. \n7. McGreal, E.P. et al., 2006, Glycobiology 16:422. \n8. Sato, K. et al., 2006, J. Biol. Chem. 281:38854. \n9. Bonkobara, M. et al., 2005, Photochem. Photobiol. 81:944. \n10. Aragane, Y. et al., 2003, J. Immunol. 171:3801.