Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Delta-1 (also known as Delta-like 1, DLL1) is an 80-100kD vertebrate homolog of the Drosophila delta protein. Rat Delta-1 is a type I transmembrane protein that is 697aa in length. It contains a 520aa extracellular domain (ECD) and a 154aa cytoplasmic region. The ECD has one DSL segment (aa158-220) and eight consecutive EGF-like domains (aa225-515). Proteolytic processing of Delta-1 by ADAMs generates soluble intracellular and extracellular domains. Delta-1 binds to Notch and limits monocyte development while promoting T cell and DC development.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, ELISA and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1-0.2ug/ml; The detection limit is 5ng/lane under non-reducing and 25ng/lane under reducing conditions.\nImmunohistochemistry: 10ug/ml detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4% PFA+14% picric acid in PBS at 4 degrees C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 min at RT. After blocking, sections were incubated with D2727-20 for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS.\nDirect ELISA: 0.5-1.0ug/ml; The detection limit is 0.3ng/well.\nFlow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in total reaction volume < 200ul. Validated using mouse splenocytes.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D2727-70

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Sheep

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes the extracellular domain of rat Delta-like 1. Species Crossreactivity: In Direct ELISA, recombinant human DDL-1: ~10%, recombinant rat Notch-1: > 1%. In Flow Cytometry, reacts with mouse.