Delta-like 1, Recombinant, Human (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Delta-like protein 1 (DLL1) is a 90-100kD type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60kD ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent g-secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).\n\nSource: Human DLL1 (Ser22-Gly 540 and His-152-Gly540; Accession # AAB61286). Contains 6X histidine tag at the C-terminus via a peptide linker. The protein was expressed in a mouse myeloma cell line, NS0.\n\nActivity: Measured by the ability of immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in MC3T3-E1 cells (Nobta M, et al. 2005. J. Biol. Chem. 280:15842). The ED50 for this effect is typically 0.25-1.0ug/ml.\n\nSDS-PAGE Analysis: 66kD and 55kD, reducing conditions. Predicted MW: 57kD and 43kD.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, aliquot and store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D2727-73

Size

50ug

Reactivities

Hum

Form

Supplied as a lyophilized powder in Tris and NaCl. Reconstitute with sterile PBS to 0.5 mg/ml.

Purity

(same/more than) 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.\nEndotoxin: <1EU by LAL method.

References

1. Gray, G.E. et al., 1999, Am. J. Pathol. 154:785. 8. Kiernan, A.E. et al., 2005, Development 132:4353. 2. Dyczynska, E. et al., 2007, J. Biol. Chem. 282:436. 9. Schmitt, T.M. and J.C. Zuniga-Pflucker, 2002, Immunity 17:749. 3. Karanu, F.N. et al., 2001, Blood 97:1960. 10. Hozumi, K. et al., 2004, Nat. Immunol. 5:638. 4. Ikeuchi, T. and S.S. Sisodia, 2003, J. Biol. Chem. 278:7751. 11. Santos, M.A. et al., 2007, Proc. Natl. Acad. Sci. 104:15454. 5. Mizuhara, E. et al., 2005, J. Biol. Chem. 280:26499. 12. Limbourg, A. et al., 2007, Circ. Res. 100:363. 6. Takahashi, Y. et al., 2003, Development 130:4259. 13. Purow, B.W. et al., 2005, Cancer Res. 65:2353. 7. Teppner, I. et al., 2007, BMC Dev. Biol. 7:68.