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Dengue Virus, Type 2 (DEN2)

Cat no: D2800-13B

Dengue Virus, Type 2 (DEN2)

Dengue infection is a major cause of morbidity in tropical and subtropical regions. It is a mosquito borne viral infection that may be asymptomatic or may cause undifferentiated fever, dengue fever, dengue haemorrhagic fever or dengue shock syndrome. The Dengue virus group consists of four closely related serotypes and belongs to the family Flaviviridae. Currently there is no vaccine or effective antiviral drugs to treat the infection.\n\nSource:\nDengue virus type 2 strain 16681 cultured in Vero cells. \n\nPreparation: \nDengue virus particles are concentrated from tissue culture supernatant by precipitation and ultracentrifugation. Antigen is purified by sucrose density gradient centrifugation. Virus particles are separated from the sucrose containing buffer by ultracentrifugation before resuspension in Medium 199. The antigen preparation is inactivated by room temperature incubation with formaldehyde. The formaldehyde is neutralized by the addition of sodium bisulphite. This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D2800-13B

Size

1ml

Applications

ELISA

Form

Supplied as a liquid in Medium 199. Preservative free. Inactivated.

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