Deoxyribonuclease I, Recombinant, Human (DNase I)

Recombinant Human Deoxyribonuclease I (rhDNase), an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarily on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2. Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments. Recombinant Human DNase produced in CHO is a glycosylated, polypeptide chain containing 260aa and having a total molecular mass of 37kD with a molecular formula of C1321H1995O396S9.\n\nSpecific Activity:\n1000IU/1mg\n\nUnit Definition: \nDnase is generally assayed according to the photometric method developed by Kunitz (see Kunitz, M., Journal of General Physiology, vol 33, p.349 and 363, 1950). One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25 degrees C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1ug of lambda phage DNA in 10 minutes at 37 degrees C in 50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3200-15

Size

10mg

Applications

ICC

Form

Supplied as a liquid in 1mM calcium chloride 150mM sodium chloride.

References

1. Biochem Genet 2005 Dec;43(11-12):629-35 2. Comp Biochem Physiol B Biochem Mol Biol 2006 Jan;143(1):70-5 3. Eur Heart J 2005 Nov;26(22):2375-80 4. Biofizika 2005 Jan-Feb;50(1):49-61 5. Mol Cells 2005 Feb 28;19(1):54-9 6. J Am Soc Nephrol 2005 Mar;16(3):697-702