Home  >  Products  >  Deoxyuridine Triphosphatase (DUT, dUTP Pyrophosphatase, dUTPase, Deoxyuridine 5'-triphosphate Nucleotidohydrolase Mitochondrial, FLJ20622)

Deoxyuridine Triphosphatase (DUT, dUTP Pyrophosphatase, dUTPase, Deoxyuridine 5'-triphosphate Nucleotidohydrolase Mitochondrial, FLJ20622)

Cat no: D3206-04H


Supplier: United States Biological
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The enzyme deoxyuridine triphosphate nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP, the immediate precursor of thymidine nucleotides. At least 3 isoforms are produced by alternative splicing. Higher mass isoform DUT-M is primarily located in the mitochondria whereas a lower mass isoform DUT-N is found in the nucleus. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1.0ug/ml Immunohistochemistry (Paraffin and Formalin): 1.0-2.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D3206-04H
Reactivities: Human, Mouse, Rat, Rabbit
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile dH20.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Alternative names: EC=3.6.1.23
Additional info: Recognizes human dUTP pyrophosphatase. Species Crossreactivity: rat, mouse, rabbit.

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