Desmocollin 3, Recombinant, Human

Desmocollin-3 is a 100-110kD type I transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1-3). The human Desmocollin-3 is synthesized with a 108 amino acid (aa) propeptide. The mature protein consists of a 555 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (4, 5). Within the ECD, human Desmocollin-3 shares 78% aa sequence identity with mouse and rat Desmocollin-3. It shares 52% and 64% aa sequence identity with Desmocollin-1 and -2, respectively. A splice variant of human Desmocollin-3 has a truncated and substituted cytoplasmic domain (4, 6). Desmocollin-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (5, 7, 8). Desmocollin-1 and -2, by contrast, are preferentially localized in the more superficial layers (7). In mouse, Desmocollin-3 is expressed throughout embryogenesis and is required for non-desmosomal functions during early development (9, 10). During apoptosis, both isoforms of Desmocollin-3 are proteolytically cleaved by caspases and metalloproteases (11). Desmocollin-3 can be upregulated or downregulated in carcinoma (6, 12, 13).\n\nSource: A DNA sequence encoding the extracellular domain of human Desmocollin-3 (DSC-3; Arg 136-Lys 690; Accession # Q14574) was fused to a 6x histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.\n\nDefinition: Measured by the ability of the immobilized protein to support the adhesion of Caki-2 (human kidney carcinoma epithelial cell line) cells.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 62.9kD protein migrates as an ~ 75-85kD protein in SDS-PAGE under reducing conditions. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3221-52B

Size

50ug

Applications

WB

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to (same/more than) 0.1mg/ml.

Purity

(same/more than) 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin (same/less than) 1EU.

References

1. Kottke, M.D. et al., 2006, J. Cell Sci. 119:797. 8. Nuber, U.A. et al., 1996, Eur. J. Cell Biol. 71:1.\n2. Garrod, D.R. et al., 2002, Mol. Memb. Biol. 19:81. 9. Chidgey, M.A.J. et al., 1997, Dev. Dyn. 210:315.\n3. Leckband, D. and A. Prakasam, 2006, Annu. Rev. Biomed. Eng. 8:259. 10. Den, Z. et al., 2006, J. Cell Sci. 119:482.\n4. Kawamura, K. et al., 1994, J. Biol. Chem. 269:26295. 11. Weiske, J. et al., 2001, J. Biol. Chem. 276:41175.\n5. King, I.A. et al., 1995, J. Invest. Dermatol. 105:314. 12. Wang, L. et al., 2007, Virchows Arch. Sept. 11 epub.\n6. Klus, G.T. et al., 2001, Int. J. Oncol. 19:169. 13. Boelens, M.C. et al., 2007, J. Clin. Pathol. 60:608.\n7. North, A.J. et al., 1996, Proc. Natl. Acad. Sci. 93:7701.