Dexras1 (Ras dexamethasone induced 1)

Dexras1 (Ras dexamethasone induced 1) belongs to the Ras superfamily of GTPases and was initially identified as a dexamethasone inducible gene (1,2). Dexras1 reportedly regulates several distinct signal transduction pathways, including MAPK signaling, NMDA receptor-nitric oxide-mediated signaling, and pathways involving adenylyl cyclases (3-5). Dexras1 can directly modulate FE65-APP-mediated transcription and regulate the photic sensitivity of the mammalian circadian clock (6,7). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \n Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3221-96T

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Kemppainen, R.J. and Behrend, E.N. (1998) J Biol Chem 273, 3129-31, 2. Tu, Y. and Wu, C. (1999) Biochim Biophys Acta 1489, 452-6, 3. Fang, M. et al. (2000) Neuron 28, 183-93, 4. Graham, T.E. et al. (2004) Biochem Biophys Res Commun 316, 307-12, 5. Nguyen, C.H. and Watts, V.J. (2005) Biochem Biophys Res Commun 332, 913-20, 6. Cheng, H.Y. et al. (2006) J Neurosci 26, 12984-95, 7. Lau, K.F. et al. (2008) J Biol Chem 283, 34728-37.

Additional Info

Recognizes human Dexras1. Cross-reacts with a 60 kDa protein of unknown origin. \n