DFF40, ID (CAD I18, DNA Fragmentation Factor, Caspase Activated Deoxyribonuclease, CPAN)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals cause the final degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562, Jurkat whole cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3224-33B

Size

50ug

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Enari, M. et al. Nature 391, 43 (1998). 2. Sakahira, H. et al. Nature 391, 96 (1998). 3. Halenbeck, R. et al. Curr. Biol. 8, 537 (1998). 4. Liu, X. et al. Proc. Natl. Acad. Sci. USA 95, 8461 (1998). 5. Mukae, N. et al. Proc Natl Acad Sci USA 95, 9123 (1998). 6. Liu, X. et al. Cell 89, 175 (1997).

Additional Info

Recognizes human DFF40. Species Crossreactivity: mouse and rat.