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DGAT1 (Diacylglycerol O-acyltransferase 1, ARGP1, DGAT, ACAT related gene product 1, ACAT-related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase homolog 1, diglyceride acyltransferase)

Cat no: 167389

DGAT1 (Diacylglycerol O-acyltransferase 1, ARGP1, DGAT, ACAT related gene product 1, ACAT-related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase homolog 1, diglyceride acyltransferase)

Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:128,000\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167389

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG

Additional Info

Recognizes mouse DGAT1 (aa67-79). Species sequence homology: Human, rat, canine, porcine, bovine.

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