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DGKI (Diacylglycerol Kinase iota, DAG Kinase iota, Diglyceride Kinase iota, DGK-iota)

DGKI (Diacylglycerol Kinase iota, DAG Kinase iota, Diglyceride Kinase iota, DGK-iota)

Cat no: D3441-87D

Supplier: United States Biological
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Diacylglycerol kinase iota (DGK-i) is an ~110kD kinase that phosphorylates the lipid diacylglycerol. DGK-i also inhibits RasGRP3 and limits Rap1 activation. It contains two zinc finger regions (aa178-232-aa251-310, a catalytic domain aa372-507, and two ankyrin repeats aa958-990-aa997-1026. Over aa925-1065, human DGK-i shares 94-96aa sequence identity with mouse and rat DGK-i, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D3441-87D
Reactivities: Human, Mouse
Hosts: Mouse
Size: 100ug
Form: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
P type: Mab
Isotype: IgG2A
Purity: Purified by Protein A/G affinity chromatography.
Additional info: Recognizes human DGK-i. Species Crossreactivity: mouse. No cross-reactivity with recombinant human DGK-a, -b, -x, -e, -h, -g, -k, -th, or -z.

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