DHEAS, Human (Dehydroepiandrosterone Sulfate) BioAssay(TM) ELISA Kit

The United States Biological DHEA ELISA is a competitive immunoenzymatic colorimetric method for quantitative determination of Dhea-s concentration in serum and plasma. \n \nDehydroepiandrosterone sulfate (DHEA-S), is a natural steroid hormone found atop of the kidneys in the human body. DHEA-S derived from enzymatic conversion of DHEA in adrenal and extradrenal tissues. DHEA-S is also produced in the gonads, adipose tissue and the brain. It is the most abundant hormone in the human body and it is precursor of all sex steroids. As most DHEA-S is produced by the zona reticularis of the adrenal, it is argued that there is a role in \nthe immune and stress response. DHEA-S may have more biologic roles. Its production in the brain suggests that is also has a role as a neurosteroid. Measurement of serum DHEA-S is a useful marker of adrenal androgen synthesis. Abnormally low levels may occur in have been reported in hypoadrenalism, while elevated levels occur in several conditions, e.g. virilizing adrenal adenoma and carcinoma, 21-hydroxylase and 3b-hydroxysteroid dehydrogenase deficiencies and in some cases of female hirsutism. Women with polycystic ovary syndrome tend to have normal or mildly elevated levels of DHEAS. As very little DHEA-S is produced \nby the gonads, measurement of DHEA-S levels may aid in the localization of androgen source in \nvirilizing conditions. DHEA-S levels show no diurnal variation. \n\nDetection Range: 0-200 ng /ml \nSample: 20ul serum \nSpecificity: ~95% \nSensitivity: 4.5 ng/ml \nTotal Time: ~75 min \n \nKit Components: \nEach kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative \n\n1. Microtiter Plate\n2. Conjugate\n3. Calibrator\n4. Positive Control\n5. Negative Control\n6. TMB\n7. Wash Buffer, 10X Diluted wash buffer to 1X\n8. Sample Diluent\n9. Stop Solution \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3228-01

Size

96Tests

Applications

ELISA

References

1. Abraham G.E., et. al., Obstet. Gynecol., 47 (4), 395 (1976) 2. Granoff A.B. et. al., Obstet. Gynecol., 53 (1), 111 (1979) 3. Hopper B.R.et. al., J. Clinic. Endocrin. Metab. 40 (3), 458 (1975) 4. Winter J.S.D, et. al., Clinic. Obstetic and Gynecol., 21 (1), 67 (1978)