Dinitrophenol (DNP)

Tissues are continually exposed to reactive oxygen species (ROS) that are produced in tissues from metabolism processes or the inflammatory response of leukocytes or macrophages. It is estimated that as much as 1% of consumed oxygen may be converted to ROS, which can cause damage to various cellular components. Proteins are one of the cellular components most vulnerable to oxidative damage by ROS, which results in an increase in protein carbonyl content. This oxidative modification of proteins can lead to cross-linking, peptide fragmentation, modified residues and the conversion of one amino group to another. If sufficient protein damage accumulates, cell death will occur.\n\nOxidative damage to proteins has been shown to result in an increased protein turnover and decreased enzymatic activity and has been associated with a number of disease states, including emphysema, arteriosclerosis and neurological disease. Oxidized proteins appear to accumulate with age and may represent 30-50% of the total proteins in aged cells. In Alzheimer's disease, oxidative damage has been shown to occur at the site of neurodegeneration.\n\nMeasurement of these carbonyl groups on proteins is a good indication of the extent of oxidative damage to proteins. Dinitrophenylhydrazine (DNPH) will react with free carbonyl groups on proteins, leading to adduction of the dinitrophenyl (DNP) group to the carbonyl group. Using an antibody to DNP, it is possible to visualize and localize the sites of oxidative damage by immunodetection of DNP adduction resulting from reaction of the tissue with DNPH.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136886

Size

100ul

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Fel, Prc

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Saiki, C., Makino, M. and Matsumoto S. Effect of 2,4-dinitrophenol under non-oxygenated condition in pons-medulla-spinal cord preparations in newborn rats: comparison with medulla- spinal cord preparations. Neurosci Lett. 2007 Feb 21;413(3):255-9.

Additional Info

Recognizes Dinitrophenol. Species Crossreactivity: human, rat, mouse, primate, porcine, bovine and feline.