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Diphtheria Toxin

Cat no: D8065-47D

Diphtheria Toxin

Diphtheria Toxin catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity. \n\nApplications: \nSuitable for use in ELISA, Fluorescence Microscopy, Western Blot, Immunocytochemistry and conjugation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D8065-47D

Size

1ml

Applications

ELISA, ICC, WB

Hosts

Goat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Pab

Purity

Purified IgG fraction to a purity of ~95%.

Isotype

IgG

References

US Biological application reference: Soria-Guerra, R.E. et al., (2009) Planta 229: 1293-1302.

Additional Info

Purified diphtheria toxin/toxoid. Demonstrated reactivity with purified toxin.

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