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DKK1, Recombinant, Human, aa32-266, His-Tag (Dickkopf-related Protein 1, DKK-1, SK)

Cat no: 137045

DKK1, Recombinant, Human, aa32-266, His-Tag (Dickkopf-related Protein 1, DKK-1, SK)

Dickkopf-related protein 1, also known as DKK1, antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. \n\nSource: \nRecombinant protein corresponding to human DKK1, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~28.2kD (258aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSTLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRH\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137045

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Ahn V.E., et al. (2011) Dev. Cell. 21:862-873. 2. Haniu M., et al. (2011) Protein Sci. 20:1802-1813.

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