DLL1 (Delta-like Protein 1, Drosophila Delta Homolog 1, Delta1, H-Delta-1, UNQ146/PRO172)

The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB). \n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul labels 1x10e6 cells in 100ul\nImmunohistochemistry: Frozen, Paraffin\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nP3U1 myeloma cells with spleen cells from Armenian hamsters.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D2727-75D

Size

100ug

Applications

IF, IHC

Reactivities

Rat

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.9% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int. Immunol. 20: 763-773. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. Int. Immunol. 21: 295-301.

Additional Info

Recognizes Delta-like protein 1 (DLL1). Species Crossreactivity: Rat.