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DNA, ds

DNA, ds

Cat no: D3878-13B

Supplier: United States Biological
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dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Paraformaldehyde fixed or frozen tissue and formalin fixed paraffin-embedded tissue sections. Optimal dilutions to be determined by the researcher. Positive Control: Tonsil tissue Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D3878-13B
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Mab
Isotype: IgG3
Purity: Purified
Alternative names: Double-stranded DNA
References: 1.Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In: Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137
Additional info: Recognizes double stranded DNA expressed in the nucleus of human cells. Produces a homogeneous staining pattern in the nucleus of normal and malignant cells.

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