Dog1 is a cell surface protein of unknown function selectively expressed in GIST. Among GIST cases with KIT mutations the Dog1 antibody identified 11% more cases than c-Kit antibody . Dog1 identifies vast majority of both c-Kit negative and PDGFRA mutated GIST cases that may still benefit from imatinib mesylate (Gleevac), an inhibitor of the kit tyrosin kinase. In addition, Dog1 immunoreactivity is seen in fewer cases of mesanchymal, epithelial tumors and melanomas when compared with c-Kit. The use of this highly sensitive and specific novel marker will increase the accuracy of GIST diagnosis.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:50. Formalin/paraffin for 20 min at RT. Staining of formalin fixed sections require heat induced antigen retrieval using EDTA, pH 8, heating to 98 degrees C for 20 min
Optimal dilutions to be determined by the researcher.
Positive Control: Gastrointestinal stromal tumors
Cellular Localization: Cytoplasmic & Membranous
Molecular Weight: 78kD
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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