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Dog1

Cat no: D8079-35

Dog1

Dog1 is a cell surface protein of unknown function selectively expressed in GIST. Among GIST cases with KIT mutations the Dog1 antibody identified 11% more cases than c-Kit antibody . Dog1 identifies vast majority of both c-Kit negative and PDGFRA mutated GIST cases that may still benefit from imatinib mesylate (Gleevac), an inhibitor of the kit tyrosin kinase. In addition, Dog1 immunoreactivity is seen in fewer cases of mesanchymal, epithelial tumors and melanomas when compared with c-Kit. The use of this highly sensitive and specific novel marker will increase the accuracy of GIST diagnosis.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:50. Formalin/paraffin for 20 min at RT. Staining of formalin fixed sections require heat induced antigen retrieval using EDTA, pH 8, heating to 98 degrees C for 20 min\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Gastrointestinal stromal tumors\n\nCellular Localization: Cytoplasmic & Membranous\n\nMolecular Weight: 78kD\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8079-35

Size

100ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Tissue culture supernatant with 15mM sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Espinosa I et al. 2008. Am J Surg Pathol. 32(2):210-218. \n2. West RB et al. 2004. Am J Pathol. 165(1): 107-113.

Additional Info

Recognizes human Dog1

Read more on Supplier website

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FC

Hosts

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Applications

FC

Hosts

Mouse

Reactivities

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More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info
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