Dopamine Beta Hydroxylase, NT (DBH, Dopamine beta Monooxygenase)

The production of neurotransmitters is controlled by environmental stimuli, which send signals through second messengers and protein kinase pathways. Regulation of gene expression of dopamine beta hydroxylase and tyrosine hydroxylase, two of the catecholamine biosynthetic enzymes, by cyclic AMP, diacyl glycerol, and Ca2+ leads to increased neurotransmitter biosynthesis. Dopamine beta-hydroxylase catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system. The disruption of the DBH gene is thought to cause changes in development and behavior in certain models. Both DBH and the multiple regulatory pathways of DBH are of increasing research in many neurological diseases.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:250-1:1000\nImmunohistochemistry: 1:250-1:1000. Staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed weak staining on both human and monkey. \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9010-11

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat, Bov, Can, NHP, Sheep

Form

Supplied as a liquid in Dulbecco

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Neuroscience Letters 120: 141-145 (1990). 2. Proceedings of 1st International Workshop on grass sickness, EMND and related disorders, 63-67 (1997). 3. Prohaska, J. and Brokate, B., Exp. Biol. Med. 226: 199-207 (2001).

Additional Info

Recognizes Dopamine Beta Hydroxylase (DbH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. 70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species Crossreactivity: By immunoblot: human, monkey (slight), sheep, canine (slight), rat and bovine. Also shown to react with equine ganglia cells.