Dopamine Receptor, D1a, D1R

Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.\n\nRat D1a receptor is a 446 aa, G-protein coupled, transmembrane receptor protein.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL\n\nELISA: 0.5-1ug/ml . Coat with 1ug control peptide/well\n\nAntibody Blocking: Use 5-10ug control peptide per 1ug antibody.\n\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: D9011-07\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9011-06

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat, Bov, Prc

Form

Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Monsama, F.J. et al., 1990, PNAS 87: 6,723-6,727. 2. Zhou, Q.Y., et al., 1990, Nature 347: 76-80. 3. Zhou, Q.Y., et al., 1992, J. Neurochem. 59: 1,875-1,883. 4. O'Dowd, B.F., et al., 1990, FEBS Lett. 262: 8-12. 5. Huang, Q., 1992, PNAS 89: 11,988-11,992. 6. Deary, A., et al., 1990, Nature 347: 72. 7. Sunahara, R.K., et al., 1990, Nature, 347: 80. 8. Ohara, K., et al., 1993, Neuropharmacol. 8: 131.

Additional Info

Recognizes dopamine receptor. The 13 aa D1R peptide showoed no significant sequence homology with other dopamine receptors (D1B, D2-D5). Species sequence homology: 100% with porcine/ bovine, 77% with human.