Dopamine Receptor D2 (DRD2)

D(2) dopamine receptor is one of five (D1 to D5) receptors for dopamine. Its activity is mediated by G proteins which inhibit adenylyl cyclase. It interacts with GPRASP1, neurabin-2, CLIC6, CADPS and CADPS2. It is a transmembrane protein that belongs to the G-protein coupled receptor 1 family. The unprocessed precursor has a length of 443 amino acids and an estimated molecular mass of 50.6kDa. Genetic variations in DRD2 may determine the genetic susceptibility to alcoholism. A missense mutation in this gene causes myoclonus dystonia and several other defects in this gene are thought to be involved in psychiatric disorders, especially in schizophrenia.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: 1:5000 on PLP fixed rat brain sections\nWestern Blot: 1:800\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9000-06G

Size

100ul

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder. Reconstitute with l00ul sterile PBS,10mg/ml BSA.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Recognizes D2 Dopamine Receptor (272-282). The reactivity of D2S Dopamine Receptor is ~70% and the reactivity of D2L Dopamine Receptor is ~60%. No crossreactivity wirth D1 Dopamine Receptor (9-21), D1 Dopamine Receptor, D3 Dopamine Receptor (2-10), D3 Dopamine Receptor, D4 Dopamine Receptor (176-185), D4 Dopamine Receptor, D5 Dopamine Receptor (23-35) and D5 Dopamine Receptor. Highly specific for this peptide sequence and is suitable for immunocytochemical detection of both the short and the long forms of the D2 dopamine receptor.