Dopamine Receptor, D2S, L, Human, Control Peptide

Control Peptide for D9016-43 (antiserum) and D9016-44 (affinity purified).\n\nDopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). \n\nDopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446aa, G-protein coupled, and transmembrane receptor protein. D2R may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains.\n\nSource: \nA 28aa synthetic peptide sequence from the human D2 Receptor, within the cytoplasmic loop # 3, coupled to KLH. The 28aa sequence is shared by both the long and short form.\n\nSequence Homology: \nHuman, bovine, canine-100%; Xenopus-50% with D2 receptors. No significant sequence homology is seen with other dopamine receptors (D1 and D3-D5).\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution: \nELISA: Coat plates with 1ug/ml.\nAntibody Blocking: Use 5-10ug per 1ug of affinity purified antibody (D9016-44) or 1ul of antiserum (D9016-45).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9016-45

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

Purity

Highly purified

References

1. Dal Toso, et al., EMBO J 8: 4025 (1989). 2. Grandy, D.K., et al., PNAS 86: 9762 (1989). 3. Monsama, F.J., et al., Nature 342: 926 (1990). 4. Giros, B., et al., Nature 342: 923 (1989). 5. Boundy, V.A., et al., Mol. Pharmacol. 43: 666-676 (1993). 6. Kamiya, T., BBRC 306: 544-549 (2003). 7. Farooqui, S.M., J. Neurochem. 57: 1363-1369 (1991). 8. Farooqui, S.M., et al., Life Sci. 51: 1509-1516 (1992). 9. Sakata, M., BBRC 575: 309-314 (1992). 10. Farooqui, S.M., BBRC 84(2): 661-667 (1992). 11. Sutoo, D., Brain Research 980: 24-30 (2003).