Dopamine Transporter (DAT)

Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long\n (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29 AA in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions. Dopamine transporter (DAT) is plasma membrane transporter for the termination of dopaminergic neurotransmission by its high affinity Na+ and Cl-reuptake of dopamine into presynaptic terminals. This protein is the target of psychomotor stimulants such as amphetamines or cocaine. DAT (human 620aa; rat/mouse 619aa glycoprotein) is predicted to contain 12 transmembrane domains with N- and C-termini in the cytoplasm.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL).\nELISA: 1:10,000-1:50,000. Coat ELISA plates with 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nD9020-17: Dopamine Transporter, Human Control Peptide \n(DAT)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9020-19

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromotography.

Isotype

IgG

References

1. Kilty, J.E., et al., Science 254: 578-579 (1991). 2. Giros, B., et al., FEBS Lett. 295: 149-154 (1991). 3. Shimada, S., et al., Science 254: 576-578 (1991). 4. Kitayama, S., et al., PNAS 89: 7782-7785 (1992). 5. Freed, C., et al., J. Comp. Neurology 359: 340-349 (1995). 6. Vaughn, R.A., et al., J. Neurochem. 66: 2146-2152 (1996). 7. Lew, R., et al., Brain Res. 584: 2666-271 (1992). 8. Lee, F. J. S., et al., The EMBO Journal (2007) 26; 2127 - 2136.

Additional Info

Recognizes human Dopamine Transporter (DAT). No significant sequence homology is seen with other transporters. Species Sequence Homology: monkey: 100%; mouse: 90%, 19/20aa; rat: 80%, 16/20aa DAT.