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ENDOG Fragment, Recombinant, Human, aa51-140, His-tag (Endonuclease G, Mitochondrial, Endo G)

ENDOG Fragment, Recombinant, Human, aa51-140, His-tag (Endonuclease G, Mitochondrial, Endo G)

Cat no: 149768

Supplier: United States Biological
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The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro. Source: Recombinant protein corresponding to human EndoG Fragment, fused to His-tag and strepII-tag at N-terminal expressed in E.coli. Molecular Weight: ~15kD Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 149768
Size: 50ug
Form: Supplied as a liquid in 1X PBS, 0.1% SDS, 0.02% sodium azide.
Purity: ~95%
References: 1. Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9. 2. Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature 2001; 412:90-4 3.Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29. 4.Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.

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