Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser52) (eIF2a)

eIF2a is a 36kD protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (a, b and g), is one of the key molecules in the initiation of translation. In mammalian cells, stress induces eIF2a to be phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2a blocks the GDP-GTP exchange activity of eIF2b, leading to inhibition of protein synthesis, growth suppression, and apoptosis induction. The phosphorylation of eIF2a is an important regulatory process in protein synthesis.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000\nOptimal dilution determined by the researcher. \n\nPositive Control: \n3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E8949-09

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Dulbecco

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Brush, M.H., et al., Mol. Cell. Biol. 23(4): 1292-1303 (2003). 2. Kimball, S.R., et al., Am. J. Physiol. Cell Physiol. 284(2): C273-C284 (2003). 3. Pataer, A., et al., Cancer Res. 62(8): 2239-2243 (2002). 4. Singleton, R.H., et al., J. Neurosci. 22(3): 791-802 (2002). 5. Soboloff, J. & Berger, S.A., J. Biol. Chem. 277(16): 13,812-13,820 (2002). 6. Vorburger, S.A., et al., Oncogene 21(41): 6278-6288 (2002). 7. Zhang, P., et al., Mol. Cell. Biol. 22(11): 3864-3874 (2002). 8. Grundmann, O., et al., J. Biol. Chem. 276(28): 25,661-25,671 (2001). 9. Li, S. & Koromilas, A.E., J. Biol. Chem. 276(17): 13,881-13,890 (2001). 10. Lu, L., et al., Mol. Cell. Biol. 21(23): 7971-7980 (2001). 11. Kumar, R., et al., J. Neurochem. 77(5): 1418-1421 (2001). 12. Patel, C.V., et al., J. Biol. Chem. 275(48): 37,993-37,998 (2000). 13. Kimball, S.R., Int. J. Biochem. Cell Biol. 31(1): 25-29 (1999).

Additional Info

Recognizes human eIF2a when phosphorylated at Ser52. Species Crossreactivity: Mouse. Species Sequence Homology: Rat, porcine, bovine, Drosophila, and yeast.