Eukaryotic Translation Initiation Factor 4E (eIF4E, eIF4EL1), phosphorylated (Ser209)

Cat no: E8949-40P

Supplier: United States Biological

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Additional Information

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25kDa) and as part of a multi-protein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation 3). There is also evidence that eIF-4E can function as an oncogene (4-5) Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:10,000 Immunohistochemistry; 1:100-250 Immunocytochemistry: 1:500 Immunoprecipitation: 1:60 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Catalogue number:	E8949-40P
Reactivities:	Human, Mouse, Rat
Hosts:	Rabbit
Applications:	Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	100ul
Form:	Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type:	Mab
Isotype:	IgG
Purity:	Supernatant
References:	1. Rychlik, et al Proc. Nat. Acad. Sci. 84: 945-949, 1987. 2. Rhoads, R. E., Joshi-Barve, S., and Rinker-Schaeffer, C.(1993) Prog. Nucleic Acids Res. Mol. Biol.46, 183-219. 3. Gert C. Scheper, Nick A. Morrice, Miranda Kleijn and Christopher G. Proud Molec. Cell. Biol.; February 2001, p. 743-754, Vol. 21, No. 3 4. Jone et al. Molec. Cell. Biol. 16: 4754-4764, 1996. 5. Wendel et al. Nature 428: 332-337, 2004.
Additional info:	Recognizes human eIF4E when phosphorylated at Ser209.

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Eukaryotic Translation Initiation Factor 4E (eIF4E, eIF4EL1), phosphorylated (Ser209)

Cat no: E8949-40P

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