Eukaryotic Translation Initiation Factor 4E (eIF4E, eIF4EL1), phosphorylated (Ser209)

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25kDa) and as part of a multi-protein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) \nmay directly regulate the rate of protein synthesis initiation 3). There is also evidence that eIF-4E can function as an oncogene (4-5) \n \nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:5000-1:10,000 \nImmunohistochemistry; 1:100-250 \nImmunocytochemistry: 1:500 \nImmunoprecipitation: 1:60 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E8949-40P

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Rychlik, et al Proc. Nat. Acad. Sci. 84: 945-949, 1987. 2. Rhoads, R. E., Joshi-Barve, S., and Rinker-Schaeffer, C.(1993) Prog. Nucleic Acids Res. Mol. Biol.46, 183-219. 3. Gert C. Scheper, Nick A. Morrice, Miranda Kleijn and Christopher G. Proud Molec. Cell. Biol.; February 2001, p. 743-754, Vol. 21, No. 3 4. Jone et al. Molec. Cell. Biol. 16: 4754-4764, 1996. 5. Wendel et al. Nature 428: 332-337, 2004.

Additional Info

Recognizes human eIF4E when phosphorylated at Ser209.