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Exosome Component 9 (EXOSC9, PMSCL1, PM/Scl-75, RRP45, Rrp45p)

Exosome Component 9 (EXOSC9, PMSCL1, PM/Scl-75, RRP45, Rrp45p)

Cat no: E9008-64A

Supplier: United States Biological
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Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. Required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:64000 Western Blot: 0.3-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell line lysates Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E9008-64A
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by affinity chromatography.
References: 1. Schilders G, Raijmakers R, Malmegrim KC, Vande Walle L, Saelens X, Vree Egberts W, van Venrooij WJ, Vandenabeele P, Pruijn GJ. Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis. Arthritis Res Ther. 2007;9(1):R12. PMID: 17280603
Additional info: Recognizes human EXOSC9 at ~75kD.

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