F1Aa (FEM1b)

Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aa and FEM1b (1,2). F1Aa/FEM1b is the homologue of C. elegans sex determining protein FEM-1 (1,2). FEM-1/F1Aa is cleaved by CED-3 and caspase (3). FEM-1/F1Aa associates with CED-4 and its mammalian homologue Apaf-1 (3). Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human and mouse tissues (1,2).\n\nApplications: \nSuitable for use in ELISA, Western Blotting. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 0.5-1ug/ml. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Mouse liver tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F0001-05

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, before the addition of 40% glycerol.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

References

1. Chan SL, Tan KO, Zhang L, Yee KS, Ronca F, Chan MY, Yu VC. F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461

Additional Info

Recognizes human F1Aa (FEM1b). An approximate 70kD band can be detected. Species crossreactivity: Human, mouse and rat.