Factor IX (HRP)

Factor IX (F.IX, Christmas Factor) is a vitamin K-dependent \nglycoprotein produced in the liver. Plasma concentration of F.IX is normally around 5ug/ml (87nM) in plasma. The biological \nimportance of F.IX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in F.IX function. In its zymogen form F.IX is a single chain molecule of 55,000 daltons. It contains two EGF-like domains and an amino-terminal domain containing 12 gamma-carboxy-glutamic acid (Gla) residues. These Gla residues allow F.IX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146- Ala147 and between Arg180-Val181. The terminal activated product in either case is F.IXab, a two-chain enzyme consisting of a heavy chain (28kD), a light chain (18kD) and an activation peptide product of 11kD. F.IX can also be cleaved into inactive products by thrombin and by elastase. The activity of F.IXab in plasma is inhibited by antithrombin and this inhibition is accelerated approximately 1000-fold in the presence of optimal concentrations of heparin(1-3). \n\nApplications:\nSuitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nRz ratio (Reinheitszahl, A403/A280): 0.47 as determined spectrophotometrically.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0017-08B

Size

100ug

Applications

ELISA

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Lawson, JH, Mann KG; Cooperative Activation of Human F.IX by the Human Extrinsic Pathway of Coagulation; J. Biol. Chem. 266, 11317-11327, (1991). 2. Enfield DL, Thompson AR; Cleavage and Activation of Factor IX by Serine Proteases; Blood 64, 821-831, (1984). 3. Limentani SA, Furie BC, Furie B, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 94-108, J.B. Lippincott Co., Philadelphia PA, USA, (1994).

Additional Info

Recognizes human Factor IX.