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Factor VII (Serum prothrombin conversion accelerator, Coagulation factor VII, SPCA, Proconvertin, F7)

Factor VII (Serum prothrombin conversion accelerator, Coagulation factor VII, SPCA, Proconvertin, F7)

Cat no: F0015-01D

Supplier: United States Biological
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Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444aa precursor. After cleavage of a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:3000 Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F0015-01D
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ul
Form: Supplied as a liquid in 0.1M Tris-glycine, pH 7.0, 0.01% thimerosal, 10% glycerol.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human F7. Species sequence homology: Mouse, monkey (91%).

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