FARP1, NT (FARP1, CDEP, PLEKHC2, FERM, RhoGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein, Pleckstrin homology domain-containing family C member 2) (Azide free) (HRP)

This gene was originally isolated through subtractive hybridization due to its increased expression in differentiated chondrocytes versus dedifferentiated chondrocytes. The resulting protein contains a predicted ezrin-like domain, a Dbl homology domain, and a pleckstrin homology domain. It is believed to be a member of the band 4.1 superfamily whose members link the cytoskeleton to the cell membrane. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

035515-HRP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Stein, J.L., et al. Neuroimage 53(3):1160-1174(2010)\nRose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :\nEvangelou, E., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (1), 220-228 (2010) :\nSugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)\nOlsen, J.V., et al. Cell 127(3):635-648(2006)

Additional Info

Human

Alternative Names

FARP1, CDEP, PLEKHC2, FERM, RhoGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein, Pleckstrin homology domain-containing family C member 2